Heterozygote X Wild-type
Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A hygromycin resistance cassette was inserted to replace exons 5 and 6. 129 background.
C (3-6 months)
129-Sirt1<tm1Mcby>
129-Sirt1<tm1Mcby>
mouse Pgk1 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Hygromycin resistance gene, mouse Pgk1 splice donor (SD), mouse Sirt1 genomic DNA
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
SirT1 knockout mice. A hygromycin resistance cassette was inserted to replace exons 5 and 6 of Sirt1 gene. Homozygous mutant mice die at birth. When outbred to CD1, SirT1 KO mice have short snouts, eyelid defect and are smaller than littermates at weaning.
<a href='https://brc.riken.jp/mus/pcr05323'>Genotyping protocol -PCR-</a>
C（3〜6か月）
Michael W. McBurney
Michael W. McBurney
RBRC05323
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
STOCK Sirt1<tm1Mcby>
条件を付加する。<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
true